Mercury induces in vivo and in vitro secretion of interleukin-1 in mice
Identifieur interne : 002C25 ( Main/Exploration ); précédent : 002C24; suivant : 002C26Mercury induces in vivo and in vitro secretion of interleukin-1 in mice
Auteurs : Johann M. Zdolsek [Suède] ; Olle Söder [Suède] ; Per Hultman [Suède]Source :
- Immunopharmacology [ 0162-3109 ] ; 1994.
English descriptors
- Teeft :
- Aqueous extracts, Arch allergy appl immunol, Autoantibody profiles, Bioassay, Biol, Biol chem, Cell activation, Cell biol, Cellbound activity, Clin immunol immunopathol, Comp biochem physiol, Contralateral, Culture medium, Cultured mouse, Cytokine, Data point, Dental implants, Dose response curves, External ears, Fetal calf serum, Genetic factors, Granholm, Guinea pigs, Hultman, Human monocytes, Immune system, Immunol, Immunological alterations inducible, Immunology today, Immunopharmacology, Incubation time, Interleukin, Lymphocyte, Lymphocyte proliferation, Macrophage, Mercuric, Mercuric chloride, Mercury compounds, Mercury treatment, Mononuclear phagocyte system, Mouse, Mouse thymocytes, Murine, Murine macrophages, Murine susceptibility, Recent studies, Secretion, Stimulatory effect, Supernatant, Test samples, Thymocyte, Thymocyte bioassay, Thymocyte proliferation assay, Unpublished observations, Zdolsek.
Abstract
Abstract: Macrophages from SJL and DBA mice incubated with mercuric chloride (HgCl2) in vitro for 24–72 h secreted an increased amount of interleukin 1 (IL-1) to the supernatant compared with control-incubated macrophages, as determined by a sensitive thymocyte proliferation assay. The increase of IL-1 activity showed a highly significant dose-response relationship, being close to that in controls at 10−8 M, and maximal after incubation with 10−5−10−6 M HgCl2 in both strains. At optimal concentrations of HgCl2 the IL-1 activity started to increase after 6 hrs incubation and reached a maximum after 48 h. Incubation with concentrations of HgCl2 higher than 10−5 M resulted in a severely reduced IL-1 activity, which correlated with a reduced cell viability. Extracts of HgCl2-incubated macrophages representing cell-bound IL-1 showed no increase in IL-1 activity, irrespective of the concentration or incubation time. Topical application of HgCl2 in a mixture of acetone-olive oil on the external ear of SJL mice induced a dose- and time-dependent increase in IL-1 activity. A maximal increase was seen after application of 1% HgCl2 for 24 h with lower IL-1 activity after 48 and 72 h. Application of 5%, but not 1% or 0.1%, slightly increased the IL-1 activity in the contralateral ear treated with acetone-olive oil only, as compared with the activity in ears from animals given no mercury treatment, suggesting a systemic effect by application of 5% HgCl2. Taken together, these in vivo and in vitro results indicate that the proinflammatory action of mercury is, at least in part, mediated by induction of IL-1.
Url:
DOI: 10.1016/0162-3109(94)90055-8
Affiliations:
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Zdolsek</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Dept. of Pathology, University of Linköping, Linköping</wicri:regionArea><wicri:noRegion>Linköping</wicri:noRegion></affiliation></author><author><name sortKey="Soder, Olle" sort="Soder, Olle" uniqKey="Soder O" first="Olle" last="Söder">Olle Söder</name><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Pediatric Endocrinology Unit, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Hultman, Per" sort="Hultman, Per" uniqKey="Hultman P" first="Per" last="Hultman">Per Hultman</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Dept. of Pathology, University of Linköping, Linköping</wicri:regionArea><wicri:noRegion>Linköping</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunopharmacology</title><title level="j" type="abbrev">IMPHAR</title><idno type="ISSN">0162-3109</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="201">201</biblScope><biblScope unit="page" to="208">208</biblScope></imprint><idno type="ISSN">0162-3109</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0162-3109</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aqueous extracts</term><term>Arch allergy appl immunol</term><term>Autoantibody profiles</term><term>Bioassay</term><term>Biol</term><term>Biol chem</term><term>Cell activation</term><term>Cell biol</term><term>Cellbound activity</term><term>Clin immunol immunopathol</term><term>Comp biochem physiol</term><term>Contralateral</term><term>Culture medium</term><term>Cultured mouse</term><term>Cytokine</term><term>Data point</term><term>Dental implants</term><term>Dose response curves</term><term>External ears</term><term>Fetal calf serum</term><term>Genetic factors</term><term>Granholm</term><term>Guinea pigs</term><term>Hultman</term><term>Human monocytes</term><term>Immune system</term><term>Immunol</term><term>Immunological alterations inducible</term><term>Immunology today</term><term>Immunopharmacology</term><term>Incubation time</term><term>Interleukin</term><term>Lymphocyte</term><term>Lymphocyte proliferation</term><term>Macrophage</term><term>Mercuric</term><term>Mercuric chloride</term><term>Mercury compounds</term><term>Mercury treatment</term><term>Mononuclear phagocyte system</term><term>Mouse</term><term>Mouse thymocytes</term><term>Murine</term><term>Murine macrophages</term><term>Murine susceptibility</term><term>Recent studies</term><term>Secretion</term><term>Stimulatory effect</term><term>Supernatant</term><term>Test samples</term><term>Thymocyte</term><term>Thymocyte bioassay</term><term>Thymocyte proliferation assay</term><term>Unpublished observations</term><term>Zdolsek</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Macrophages from SJL and DBA mice incubated with mercuric chloride (HgCl2) in vitro for 24–72 h secreted an increased amount of interleukin 1 (IL-1) to the supernatant compared with control-incubated macrophages, as determined by a sensitive thymocyte proliferation assay. The increase of IL-1 activity showed a highly significant dose-response relationship, being close to that in controls at 10−8 M, and maximal after incubation with 10−5−10−6 M HgCl2 in both strains. At optimal concentrations of HgCl2 the IL-1 activity started to increase after 6 hrs incubation and reached a maximum after 48 h. Incubation with concentrations of HgCl2 higher than 10−5 M resulted in a severely reduced IL-1 activity, which correlated with a reduced cell viability. Extracts of HgCl2-incubated macrophages representing cell-bound IL-1 showed no increase in IL-1 activity, irrespective of the concentration or incubation time. Topical application of HgCl2 in a mixture of acetone-olive oil on the external ear of SJL mice induced a dose- and time-dependent increase in IL-1 activity. A maximal increase was seen after application of 1% HgCl2 for 24 h with lower IL-1 activity after 48 and 72 h. Application of 5%, but not 1% or 0.1%, slightly increased the IL-1 activity in the contralateral ear treated with acetone-olive oil only, as compared with the activity in ears from animals given no mercury treatment, suggesting a systemic effect by application of 5% HgCl2. Taken together, these in vivo and in vitro results indicate that the proinflammatory action of mercury is, at least in part, mediated by induction of IL-1.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><noRegion><name sortKey="Zdolsek, Johann M" sort="Zdolsek, Johann M" uniqKey="Zdolsek J" first="Johann M." last="Zdolsek">Johann M. Zdolsek</name></noRegion><name sortKey="Hultman, Per" sort="Hultman, Per" uniqKey="Hultman P" first="Per" last="Hultman">Per Hultman</name><name sortKey="Soder, Olle" sort="Soder, Olle" uniqKey="Soder O" first="Olle" last="Söder">Olle Söder</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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